Počet záznamů: 1

Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology

  1. 1.
    0354405 - FGU-C 2011 RIV US eng J - Článek v odborném periodiku
    Burdíková, Zuzana - Čapek, Martin - Ostašov, Pavel - Machač, Jiří - Pelc, Radek - Mitchell, E.A.D. - Kubínová, Lucie
    Testate Amoebae Examined by Confocal and Two-Photon Microscopy: Implications for Taxonomy and Ecophysiology.
    Microscopy and Microanalysis. Roč. 16, č. 6 (2010), s. 735-746 ISSN 1431-9276
    Grant CEP: GA MŠk(CZ) LC06063; GA ČR(CZ) GA102/08/0691
    Výzkumný záměr: CEZ:AV0Z50110509; CEZ:AV0Z60050516
    Klíčová slova: testate amoebae * confocal microscopy * environmental scanning electron microscopy
    Kód oboru RIV: JD - Využití počítačů, robotika a její aplikace
    Impakt faktor: 2.179, rok: 2010

    In our study we applied both traditional and advanced microscopic imaging techniques to study testate amoebae (TA) shell and cell morphology. When compared with scanning electron microscopy (SEM) or environmental SEM (ESEM), confocal laser scanning microscopy (CLSM) and two-photon excitation microscopy (TPEM) offered several additional advantages for investigations requiring information of morphological characteristics, useful for taxonomical and ecophysiological research: (1) CLSM and TPEM allow imaging of inner structures of TA in contrast to SEM or ESEM. (2) CLSM and TPEM can simplify and refine studies of morphology. (3) CLSM and TPEM allow us to obtain data that can be used for stereological estimations of volumes of TA. (4) Fluorescence markers allow us to see structures not visible in transmitting light
    Trvalý link: http://hdl.handle.net/11104/0193414