Počet záznamů: 1

Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics

  1. 1.
    0351177 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
    Rey, M. - Mrázek, H. - Pompach, Petr - Novák, P. - Pelosi, L. - Brandolin, G. - Forest, E. - Havlíček, Vladimír - Man, P.
    Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics.
    Analytical Chemistry. Roč. 82, č. 12 (2010), s. 5107-5116 ISSN 0003-2700
    Grant CEP: GA AV ČR KJB500200612; GA MŠk LC545
    Výzkumný záměr: CEZ:AV0Z50200510
    Klíčová slova: mass spectrometry * proteomics * peptides
    Kód oboru RIV: CE - Biochemie
    Impakt faktor: 5.874, rok: 2010

    Paper describes novel method suitable for removal of non-ionic POE-based detergents and PEG or PPG polymers. The removal is done as solid phase extraction with the peptides bound to the reversed phase resin. The detergent or polymer molecules are washed away using chlorinated solvents. Several aspects of the method were carefully tested and its sensitivity was shown. It is suitable for removal of different non-ionic detergents or polymers on peptide or protein level and it can be easily automated. Its compatibility with hydrogen/deuterium exchange was shown and demonstrated on the mapping of full membrane protein
    Trvalý link: http://hdl.handle.net/11104/0190984