Počet záznamů: 1
Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics
- 1. 0351177 - MBU-M 2011 RIV US eng J - Článek v odborném periodiku
Rey, M. - Mrázek, H. - Pompach, Petr - Novák, P. - Pelosi, L. - Brandolin, G. - Forest, E. - Havlíček, Vladimír - Man, P.
Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics.
Analytical Chemistry. Roč. 82, č. 12 (2010), s. 5107-5116. ISSN 0003-2700
Grant CEP: GA AV ČR KJB500200612; GA MŠk LC545
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: mass spectrometry * proteomics * peptides
Kód oboru RIV: CE - Biochemie
Impakt faktor: 5.874, rok: 2010
Paper describes novel method suitable for removal of non-ionic POE-based detergents and PEG or PPG polymers. The removal is done as solid phase extraction with the peptides bound to the reversed phase resin. The detergent or polymer molecules are washed away using chlorinated solvents. Several aspects of the method were carefully tested and its sensitivity was shown. It is suitable for removal of different non-ionic detergents or polymers on peptide or protein level and it can be easily automated. Its compatibility with hydrogen/deuterium exchange was shown and demonstrated on the mapping of full membrane protein
Trvalý link: http://hdl.handle.net/11104/0190984