Počet záznamů: 1  

Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension

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    SYSNO ASEP0555843
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevCap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension
    Tvůrce(i) Dahale, S. (GB)
    Ruiz-Orera, J. (DE)
    Šilhavý, Jan (FGU-C) RID, ORCID
    Hübner, N. (DE)
    van Heesch, S. (NL)
    Pravenec, Michal (FGU-C) RID, ORCID
    Atanur, S. S. (GB)
    Číslo článkue202101234
    Zdroj.dok.Life Science Alliance
    Roč. 5, č. 4 (2022)
    Poč.str.13 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovacap analysis of gene expression (CAGE) ; alternative promoter usage ; spontaneously hypertensive rat ; left ventricle
    Obor OECDBiochemistry and molecular biology
    Způsob publikováníOpen access
    Institucionální podporaFGU-C - RVO:67985823
    UT WOS000742638600001
    EID SCOPUS85123269301
    DOI10.26508/lsa.202101234
    AnotaceThe role of alternative promoter usage in tissue-specific gene expression has been well established, however, its role in complex diseases is poorly understood. We performed cap analysis of gene expression (CAGE) sequencing from the left ventricle of a rat model of hypertension, the spontaneously hypertensive rat (SHR), and a normotensive strain, Brown Norway to understand the role of alternative promoter usage in complex disease. We identified 26,560 CAGE-defined transcription start sites in the rat left ventricle, including 1,970 novel cardiac transcription start sites. We identified 28 genes with alternative promoter usage between SHR and Brown Norway, which could lead to protein isoforms differing at the amino terminus between two strains and 475 promoter switching events altering the length of the 5′ UTR. We found that the shift in Insr promoter usage was significantly associated with insulin levels and blood pressure within a panel of HXB/BXH recombinant inbred rat strains, suggesting that hyperinsulinemia due to insulin resistance might lead to hypertension in SHR. Our study provides a preliminary evidence of alternative promoter usage in complex diseases.
    PracovištěFyziologický ústav
    KontaktLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Rok sběru2023
    Elektronická adresahttps://doi.org/10.26508/lsa.202101234
Počet záznamů: 1