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Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension

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    0555843 - FGÚ 2023 RIV US eng J - Článek v odborném periodiku
    Dahale, S. - Ruiz-Orera, J. - Šilhavý, Jan - Hübner, N. - van Heesch, S. - Pravenec, Michal - Atanur, S. S.
    Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension.
    Life Science Alliance. Roč. 5, č. 4 (2022), č. článku e202101234. E-ISSN 2575-1077
    Grant ostatní: AV ČR(CZ) AP1502
    Program: Akademická prémie - Praemium Academiae
    Institucionální podpora: RVO:67985823
    Klíčová slova: cap analysis of gene expression (CAGE) * alternative promoter usage * spontaneously hypertensive rat * left ventricle
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 4.591, rok: 2020
    Způsob publikování: Open access
    https://doi.org/10.26508/lsa.202101234

    The role of alternative promoter usage in tissue-specific gene expression has been well established, however, its role in complex diseases is poorly understood. We performed cap analysis of gene expression (CAGE) sequencing from the left ventricle of a rat model of hypertension, the spontaneously hypertensive rat (SHR), and a normotensive strain, Brown Norway to understand the role of alternative promoter usage in complex disease. We identified 26,560 CAGE-defined transcription start sites in the rat left ventricle, including 1,970 novel cardiac transcription start sites. We identified 28 genes with alternative promoter usage between SHR and Brown Norway, which could lead to protein isoforms differing at the amino terminus between two strains and 475 promoter switching events altering the length of the 5′ UTR. We found that the shift in Insr promoter usage was significantly associated with insulin levels and blood pressure within a panel of HXB/BXH recombinant inbred rat strains, suggesting that hyperinsulinemia due to insulin resistance might lead to hypertension in SHR. Our study provides a preliminary evidence of alternative promoter usage in complex diseases.
    Trvalý link: http://hdl.handle.net/11104/0330305

     
     
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