Počet záznamů: 1
Synthetic antimicrobial peptides of the halictines family disturb the membrane integrity of Candida cells
- 1.
SYSNO ASEP 0476797 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Synthetic antimicrobial peptides of the halictines family disturb the membrane integrity of Candida cells Tvůrce(i) Kodedová, Marie (FGU-C) RID, ORCID
Sychrová, Hana (FGU-C) RID, ORCIDZdroj.dok. Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736
Roč. 1859, č. 10 (2017), s. 1851-1858Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova antimicrobial peptide ; Candida ; diS-C3(3) assay ; membrane potential ; membrane lipids ; halictine Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP TA04010638 GA TA ČR - Technologická agentura ČR GA16-03398S GA ČR - Grantová agentura ČR LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000411419000010 EID SCOPUS 85024090021 DOI 10.1016/j.bbamem.2017.06.005 Anotace We compared the potency of four derivatives of the antimicrobial peptide halictine-2 against six Candida species. Observed activity was peptide and species specific. Halictines rapidly permeabilized cell membranes and caused the leakage of cytosolic components. Their killing potential was enhanced by the commercial antimicrobial agent octenidine dihydrochloride. The effect on C. glabrata cells did not depend on the activity of Cdr pumps, but was influenced by their lipid composition. The pre-treatment of cells with myriocin, an inhibitor of sphingolipid synthesis, enhanced the peptides' activity, whereas pre-treatment with terbinafine and fluconazole, inhibitors of sterol synthesis, significantly weakened their efficacy. The killing efficacy of peptides increased in combination with amphotericin B. Thus the mode of action of halictines is likely to depend on the plasma-membrane sterols, which might explain the observed differences among the tested Candida species. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2018
Počet záznamů: 1