Počet záznamů: 1  

Synthetic antimicrobial peptides of the halictines family disturb the membrane integrity of Candida cells

  1. 1.
    0476797 - FGU-C 2018 RIV NL eng J - Článek v odborném periodiku
    Kodedová, Marie - Sychrová, Hana
    Synthetic antimicrobial peptides of the halictines family disturb the membrane integrity of Candida cells.
    Biochimica Et Biophysica Acta-Biomembranes. Roč. 1859, č. 10 (2017), s. 1851-1858. ISSN 0005-2736
    Grant CEP: GA TA ČR(CZ) TA04010638; GA ČR(CZ) GA16-03398S; GA MŠk(CZ) LQ1604; GA MŠk(CZ) ED1.1.00/02.0109
    Institucionální podpora: RVO:67985823
    Klíčová slova: antimicrobial peptide * Candida * diS-C3(3) assay * membrane potential * membrane lipids * halictine
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Obor OECD: Microbiology
    Impakt faktor: 3.438, rok: 2017

    We compared the potency of four derivatives of the antimicrobial peptide halictine-2 against six Candida species. Observed activity was peptide and species specific. Halictines rapidly permeabilized cell membranes and caused the leakage of cytosolic components. Their killing potential was enhanced by the commercial antimicrobial agent octenidine dihydrochloride. The effect on C. glabrata cells did not depend on the activity of Cdr pumps, but was influenced by their lipid composition. The pre-treatment of cells with myriocin, an inhibitor of sphingolipid synthesis, enhanced the peptides' activity, whereas pre-treatment with terbinafine and fluconazole, inhibitors of sterol synthesis, significantly weakened their efficacy. The killing efficacy of peptides increased in combination with amphotericin B. Thus the mode of action of halictines is likely to depend on the plasma-membrane sterols, which might explain the observed differences among the tested Candida species.
    Trvalý link: http://hdl.handle.net/11104/0273210