Počet záznamů: 1

The molecular mass of dextran used to modify magnetite nanoparticles affects insulin amyloid aggregation

SYSNO ASEP	0474865
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The molecular mass of dextran used to modify magnetite nanoparticles affects insulin amyloid aggregation
Tvůrce(i)	Sipošová, K. (SK) Pospíšková, K. (CZ) Bednáriková, Z. (SK) Šafařík, Ivo (BC-A)_{RID, ORCID} Šafaříková, Miroslava (BC-A)_RID Kubovčíková, M. (SK) Kopčanský, P. (SK) Gázová, Z. (SK)
Zdroj.dok.	Journal of Magnetism and Magnetic Materials. - : Elsevier - ISSN 0304-8853 Roč. 427, April (2017), s. 48-53
Poč.str.	6 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	amyloid aggregation ; nanoparticles ; magnetic fluid ; dextran ; insulin
Vědní obor RIV	CE - Biochemie
Obor OECD	Biochemistry and molecular biology
Institucionální podpora	BC-A - RVO:60077344
UT WOS	000397199400010
EID SCOPUS	85006049767
DOI	10.1016/j.jmmm.2016.10.083
Anotace	Protein transformation from its soluble state into amyloid aggregates is associated with amyloid-related diseases. Amyloid deposits of insulin fibrils have been found in the sites of subcutaneous insulin application in patients with prolonged diabetes. Using atomic force microscopy and ThT fluorescence assay we have investigated the interference of insulin amyloid aggregation with superparamagnetic Fe3O4-based nanoparticles (SPIONs) coated with dextran (DEX), molecular mass of dextran was equal to 15-20, 40 or 70 kDa. The obtained data indicate that all three types of dextran coated nanoparticles (NP-FeDEXs) are able to inhibit insulin fibrillization and to destroy amyloid fibrils. The extent of anti-amyloid activities depends on the properties of NP-FeDEXs, mainly on the size of nanoparticles which is determined by molecular mass of dextran molecules. The most effective inhibiting activity was observed for the smallest nanoparticles coated with 1520 kDa dextran. Contrary, the highest destroying activity was observed for the largest NP-FeDEX (70 kDa dextran).
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2018

Počet záznamů: 1