The molecular mass of dextran used to modify magnetite nanoparticles affects insulin amyloid aggregation

0474865 - BC 2018 RIV NL eng J - Článek v odborném periodiku
Sipošová, K. - Pospíšková, K. - Bednáriková, Z. - Šafařík, Ivo - Šafaříková, Miroslava - Kubovčíková, M. - Kopčanský, P. - Gázová, Z.
The molecular mass of dextran used to modify magnetite nanoparticles affects insulin amyloid aggregation.
Journal of Magnetism and Magnetic Materials. Roč. 427, April (2017), s. 48-53. ISSN 0304-8853. E-ISSN 1873-4766
Institucionální podpora: RVO:60077344
Klíčová slova: amyloid aggregation * nanoparticles * magnetic fluid * dextran * insulin
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.046, rok: 2017

Protein transformation from its soluble state into amyloid aggregates is associated with amyloid-related diseases. Amyloid deposits of insulin fibrils have been found in the sites of subcutaneous insulin application in patients with prolonged diabetes. Using atomic force microscopy and ThT fluorescence assay we have investigated the interference of insulin amyloid aggregation with superparamagnetic Fe3O4-based nanoparticles (SPIONs) coated with dextran (DEX), molecular mass of dextran was equal to 15-20, 40 or 70 kDa. The obtained data indicate that all three types of dextran coated nanoparticles (NP-FeDEXs) are able to inhibit insulin fibrillization and to destroy amyloid fibrils. The extent of anti-amyloid activities depends on the properties of NP-FeDEXs, mainly on the size of nanoparticles which is determined by molecular mass of dextran molecules. The most effective inhibiting activity was observed for the smallest nanoparticles coated with 1520 kDa dextran. Contrary, the highest destroying activity was observed for the largest NP-FeDEX (70 kDa dextran).
Trvalý link: http://hdl.handle.net/11104/0277687