Počet záznamů: 1
Seasonal variation and distribution of total and active microbial community of beta-glucosidase encoding genes in coniferous forest soil
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SYSNO ASEP 0474445 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Seasonal variation and distribution of total and active microbial community of beta-glucosidase encoding genes in coniferous forest soil Tvůrce(i) Pathan, S.I. (IT)
Žifčáková, Lucia (MBU-M) RID
Ceccherini, M.T. (IT)
Pantani, O.L. (IT)
Větrovský, Tomáš (MBU-M) ORCID, RID
Baldrian, Petr (MBU-M) RID, ORCIDZdroj.dok. Soil Biology and Biochemistry. - : Elsevier - ISSN 0038-0717
Roč. 105, February (2017), s. 71-80Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Beta-Glucosidases ; Forest soil ; Bacteria Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA16-08916S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000392788600010 EID SCOPUS 84997293631 DOI 10.1016/j.soilbio.2016.11.003 Anotace Cellulose is the most abundant polysaccharide in the dead plant biomass, and its degradation is an important part of global carbon cycle. Beta-Glucosidases complete the final step of cellulose hydrolysis by converting cellobiose to glucose. Genetic potential and expression of Beta-glucosidase genes were studied in the topsoil of a Picea abies forest in two contrasting seasons. These seasons were the summer, representing the peak of plant photosynthetic activity, and late winter, after an extended period with no photosynthate input. Fungal and bacterial beta-glucosidase genes belonging to glycoside hydrolase families GH1 and GH3 amplified from DNA and RNA and amplicon pools were analyzed. Transcript pool were largely corresponded to gene pools, although some abundant transcripts were not found in the gene pool. The major reservoirs of Beta-glucosidase genes were the fungal phylum Ascomycota and Basidiomycota and the bacterial phyla Firmicutes, Actinobacteria, Proteobacteria Acidobacteria and Deinococcus-Thermus. This indicates that a diverse microbial community utilized cellobiose. Seasonality influenced both genetic diversity of Beta-glucosidase genes and their expression. The results indicate that a complex community of bacteria and fungi expresses beta-glucosidases in forest soils. Even Beta-glucosidase genes showing low abundance in DNA may be functionally important as revealed by their high expression especially in basidiomycota. The functional diversity in the studied ecosystem clearly exhibited a seasonal pattern. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1