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The small 6C RNA of Corynebacterium glutamicum is involved in the SOS response
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SYSNO ASEP 0467887 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The small 6C RNA of Corynebacterium glutamicum is involved in the SOS response Tvůrce(i) Pahlke, J. (DE)
Dostálová, Hana (MBU-M)
Holátko, Jiří (MBU-M)
Degner, U. (DE)
Bott, M. (DE)
Pátek, Miroslav (MBU-M) RID, ORCID
Polen, T. (DE)Zdroj.dok. RNA biology. - : Taylor & Francis - ISSN 1547-6286
Roč. 13, č. 9 (2016), s. 848-860Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Actinobacteria ; branched morphology ; cell division Vědní obor RIV EE - Mikrobiologie, virologie Institucionální podpora MBU-M - RVO:61388971 UT WOS 000383358100013 EID SCOPUS 84980022763 DOI 10.1080/15476286.2016.1205776 Anotace The 6C RNA family is a class of small RNAs highly conserved in Actinobacteria, including the genera Mycobacterium, Streptomyces and Corynebacterium whose physiological function has not yet been elucidated. We found that strong transcription of the cgb_03605 gene, which encodes 6C RNA in C. glutamicum, was driven by the SigA- and SigB-dependent promoter P-cgb_03605. 6C RNA was detected at high level during exponential growth phase (180 to 240 molcules per cell) which even increased at the entry of the stationary phase. 6C RNA level did not decrease within 240min after transcription had been stopped with rifampicin, which suggests high 6C RNA stability. The expression of cgb_03605 further increased approximately twofold in the presence of DNA-damaging mitomycin C (MMC) and nearly threefold in the absence of LexA. Deletion of the 6C RNA gene cgb_03605 resulted in a higher sensitivity of C. glutamicum toward MMC and UV radiation. These results indicate that 6C RNA is involved in the DNA damage response. Both 6C RNA level-dependent pausing of cell growth and branched cell morphology in response to MMC suggest that 6C RNA may also be involved in a control of cell division. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
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