Počet záznamů: 1  

The small 6C RNA of Corynebacterium glutamicum is involved in the SOS response

  1. 1. 0467887 - MBU-M 2017 RIV US eng J - Článek v odborném periodiku
    Pahlke, J. - Dostálová, Hana - Holátko, Jiří - Degner, U. - Bott, M. - Pátek, Miroslav - Polen, T.
    The small 6C RNA of Corynebacterium glutamicum is involved in the SOS response.
    RNA biology. Roč. 13, č. 9 (2016), s. 848-860. ISSN 1547-6286
    Institucionální podpora: RVO:61388971
    Klíčová slova: Actinobacteria * branched morphology * cell division
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 3.900, rok: 2016

    The 6C RNA family is a class of small RNAs highly conserved in Actinobacteria, including the genera Mycobacterium, Streptomyces and Corynebacterium whose physiological function has not yet been elucidated. We found that strong transcription of the cgb_03605 gene, which encodes 6C RNA in C. glutamicum, was driven by the SigA- and SigB-dependent promoter P-cgb_03605. 6C RNA was detected at high level during exponential growth phase (180 to 240 molcules per cell) which even increased at the entry of the stationary phase. 6C RNA level did not decrease within 240min after transcription had been stopped with rifampicin, which suggests high 6C RNA stability. The expression of cgb_03605 further increased approximately twofold in the presence of DNA-damaging mitomycin C (MMC) and nearly threefold in the absence of LexA. Deletion of the 6C RNA gene cgb_03605 resulted in a higher sensitivity of C. glutamicum toward MMC and UV radiation. These results indicate that 6C RNA is involved in the DNA damage response. Both 6C RNA level-dependent pausing of cell growth and branched cell morphology in response to MMC suggest that 6C RNA may also be involved in a control of cell division.
    Trvalý link: http://hdl.handle.net/11104/0266191