Počet záznamů: 1

Molecular identification of Entamoeba species in savanna woodland chimpanzees (Pan troglodytes schweinfurthii)

SYSNO ASEP	0460290
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Molecular identification of Entamoeba species in savanna woodland chimpanzees (Pan troglodytes schweinfurthii)
Tvůrce(i)	Jirků-Pomajbíková, K. (CZ) Čepička, I. (CZ) Kalousová, B. (CZ) Jirků, M. (CZ) Stewart, F. (GB) Levecke, B. (BE) Modrý, D. (CZ) Piel, A. K. (GB) Petrželková, Klára Judita (UBO-W)_{RID, SAI, ORCID}
Celkový počet autorů	9
Zdroj.dok.	Parasitology. - : Cambridge University Press - ISSN 0031-1820 Roč. 143, č. 6 (2016), s. 741-748
Poč.str.	8 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Entamoeba ; molecular diversity ; great apes ; chimpanzee ; savannah
Vědní obor RIV	EG - Zoologie
CEP	GA206/09/0927 GA ČR - Grantová agentura ČR
Institucionální podpora	UBO-W - RVO:68081766
UT WOS	000376783000008
EID SCOPUS	84960108987
DOI	10.1017/S0031182016000263
Anotace	To address the molecular diversity and occurrence of pathogenic species of the genus Entamoeba spp. in wild non-human primates (NHP) we conducted molecular-phylogenetic analyses on Entamoeba from wild chimpanzees living in the Issa Valley, Tanzania. We compared the sensitivity of molecular [using a genus-specific polymerase chain reaction (PCR)] and coproscopic detection (merthiolate-iodine-formaldehyde concentration) of Entamoeba spp. We identified Entamoeba spp. in 72 chimpanzee fecal samples (79%) subjected to species-specific PCRs for six Entamoeba species/groups (Entamoeba histolytica, Entamoeba nuttalli, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba coli and Entamoeba polecki ST2). We recorded three Entamoeba species: E. coli (47%), E. dispar (16%), Entamoeba hartmanni (51%). Coproscopically, we could only distinguish the cysts of complex E. histolytica/dispar/moshkovskii/nuttalli and E. coli. Molecular prevalence of entamoebas was higher than the prevalence based on the coproscopic examination. Our molecular phylogenies showed that sequences of E. dispar and E. coli from Issa chimpanzees are closely related to sequences from humans and other NHP from GenBank. The results showed that wild chimpanzees harbour Entamoeba species similar to those occurring in humans; however, no pathogenic species were detected. Molecular-phylogenetic methods are critical to improve diagnostics of entamoebas in wild NHP and for determining an accurate prevalence of Entamoeba species.
Pracoviště	Ústav biologie obratlovců
Kontakt	Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524
Rok sběru	2017

Počet záznamů: 1