Počet záznamů: 1  

Molecular identification of Entamoeba species in savanna woodland chimpanzees (Pan troglodytes schweinfurthii)

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    0460290 - ÚBO 2017 RIV GB eng J - Článek v odborném periodiku
    Jirků-Pomajbíková, K. - Čepička, I. - Kalousová, B. - Jirků, M. - Stewart, F. - Levecke, B. - Modrý, D. - Piel, A. K. - Petrželková, Klára Judita
    Molecular identification of Entamoeba species in savanna woodland chimpanzees (Pan troglodytes schweinfurthii).
    Parasitology. Roč. 143, č. 6 (2016), s. 741-748. ISSN 0031-1820. E-ISSN 1469-8161
    Grant CEP: GA ČR GA206/09/0927
    Institucionální podpora: RVO:68081766
    Klíčová slova: Entamoeba * molecular diversity * great apes * chimpanzee * savannah
    Kód oboru RIV: EG - Zoologie
    Impakt faktor: 2.620, rok: 2016

    To address the molecular diversity and occurrence of pathogenic species of the genus Entamoeba spp. in wild non-human primates (NHP) we conducted molecular-phylogenetic analyses on Entamoeba from wild chimpanzees living in the Issa Valley, Tanzania. We compared the sensitivity of molecular [using a genus-specific polymerase chain reaction (PCR)] and coproscopic detection (merthiolate-iodine-formaldehyde concentration) of Entamoeba spp. We identified Entamoeba spp. in 72 chimpanzee fecal samples (79%) subjected to species-specific PCRs for six Entamoeba species/groups (Entamoeba histolytica, Entamoeba nuttalli, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba coli and Entamoeba polecki ST2). We recorded three Entamoeba species: E. coli (47%), E. dispar (16%), Entamoeba hartmanni (51%). Coproscopically, we could only distinguish the cysts of complex E. histolytica/dispar/moshkovskii/nuttalli and E. coli. Molecular prevalence of entamoebas was higher than the prevalence based on the coproscopic examination. Our molecular phylogenies showed that sequences of E. dispar and E. coli from Issa chimpanzees are closely related to sequences from humans and other NHP from GenBank. The results showed that wild chimpanzees harbour Entamoeba species similar to those occurring in humans; however, no pathogenic species were detected. Molecular-phylogenetic methods are critical to improve diagnostics of entamoebas in wild NHP and for determining an accurate prevalence of Entamoeba species.
    Trvalý link: http://hdl.handle.net/11104/0260406

     
     
Počet záznamů: 1  

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