Počet záznamů: 1

Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography

SYSNO ASEP	0460139
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography
Tvůrce(i)	Flodrová, Dana (UIACH-O) Bobálová, Janette (UIACH-O)_{RID, ORCID} Laštovičková, Markéta (UIACH-O)_{RID, ORCID}
Celkový počet autorů	3
Zdroj.dok.	Cereal Research Communications - ISSN 0133-3720 Roč. 44, č. 2 (2016), s. 286-297
Poč.str.	12 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	HU - Maďarsko
Klíč. slova	barley ; wheat ; glycoprotein ; mass spectrometry ; lectin chromatography
Vědní obor RIV	CB - Analytická chemie, separace
CEP	GPP503/12/P395 GA ČR - Grantová agentura ČR
Institucionální podpora	UIACH-O - RVO:68081715
UT WOS	000388000600010
EID SCOPUS	84971406539
DOI	10.1556/0806.44.2016.019
Anotace	Research of cereal glycoproteins is important for understanding of their functional properties, their role during technological processing of cereals and it can serve as a valuable tool for the detection of cereal allergens. The main intention of this study was the screening of profile of water-soluble glycoproteins present in barley (Hordeum vulgare), wheat durum (Triticum durum) and spelt (Triticum spelta). Lectin monolithic HPLC column was used for rapid and effective enrichment of glycoprotein fractions. Captured glycoproteins were electrophoretically separated and analyzed by MALDI-TOF MS. Presented procedure resulted in identification of a group of N-glycoprotein candidates with affinity to lectin concanavalin A (ConA). Such molecules could have, among others, an allergenic potential.
Pracoviště	Ústav analytické chemie
Kontakt	Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
Rok sběru	2017

Počet záznamů: 1