Počet záznamů: 1
Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography
- 1.0460139 - UIACH-O 2017 RIV HU eng J - Článek v odborném periodiku
Flodrová, Dana - Bobálová, Janette - Laštovičková, Markéta
Cereal n-glycoproteins enrichment by lectin affinity monolithic chromatography.
Cereal Research Communications. Roč. 44, č. 2 (2016), s. 286-297. ISSN 0133-3720
Grant CEP: GA ČR(CZ) GPP503/12/P395
Institucionální podpora: RVO:68081715
Klíčová slova: barley * wheat * glycoprotein * mass spectrometry * lectin chromatography
Kód oboru RIV: CB - Analytická chemie, separace
Impakt faktor: 0.496, rok: 2016
Research of cereal glycoproteins is important for understanding of their functional properties, their role during technological processing of cereals and it can serve as a valuable tool for the detection of cereal allergens. The main intention of this study was the screening of profile of water-soluble glycoproteins present in barley (Hordeum vulgare), wheat durum (Triticum durum) and spelt (Triticum spelta). Lectin monolithic HPLC column was used for rapid and effective enrichment of glycoprotein fractions. Captured glycoproteins were electrophoretically separated and analyzed by MALDI-TOF MS. Presented procedure resulted in identification of a group of N-glycoprotein candidates with affinity to lectin concanavalin A (ConA). Such molecules could have, among others, an allergenic potential.
Trvalý link: http://hdl.handle.net/11104/0260261