Počet záznamů: 1
Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity
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SYSNO ASEP 0458566 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity Tvůrce(i) Zasońska, Beata Anna (UMCH-V) RID, ORCID
Líšková, A. (SK)
Kuricová, M. (SK)
Tulinská, J. (SK)
Pop-Georgievski, Ognen (UMCH-V) RID, ORCID
Čiampor, F. (SK)
Vávra, I. (SK)
Dušinská, M. (NO)
Ilavská, S. (SK)
Horváthová, M. (SK)
Horák, Daniel (UMCH-V) RID, ORCIDZdroj.dok. Croatian Medical Journal - ISSN 0353-9504
Roč. 57, č. 2 (2016), s. 165-178Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. HR - Chorvatsko Klíč. slova core-shell maghemite nanoparticles ; proliferative activity of lymphocytes ; phagocytic activity Vědní obor RIV CD - Makromolekulární chemie CEP GC16-01128J GA ČR - Grantová agentura ČR Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000377053400009 EID SCOPUS 84971543394 Anotace To determine cytotoxicity and effect of silica-coated magnetic nanoparticles (MNPs) on immune response, in particular lymphocyte proliferative activity, phagocytic activity, and leukocyte respiratory burst and in vitro production of interleukin-6 (IL-6) and 8 (IL-8), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and granulocyte macrophage colony stimulating factor (GM-CSF). Maghemite was prepared by coprecipitation of iron salts with ammonia, oxidation with NaOCl and modified by tetramethyl orthosilicate and aminosilanes. Particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and X-ray photoelectron spectroscopy (XPS). Cytotoxicity and lymphocyte proliferative activity were assessed using [H-3]-thymidine incorporation into DNA of proliferating human peripheral blood cells. Phagocytic activity and leukocyte respiratory burst were measured by flow cytometry; cytokine levels in cell supernatants were determined by ELISA. The cytotoxicity and in vitro immunotoxicity were found to be minimal in the newly developed porous core-shell gamma-Fe2O3&SiO2-NH2 magnetic nanoparticles. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2017
Počet záznamů: 1