Počet záznamů: 1  

Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity

  1. 1. 0458566 - UMCH-V 2017 RIV HR eng J - Článek v odborném periodiku
    Zasońska, Beata Anna - Líšková, A. - Kuricová, M. - Tulinská, J. - Pop-Georgievski, Ognen - Čiampor, F. - Vávra, I. - Dušinská, M. - Ilavská, S. - Horváthová, M. - Horák, Daniel
    Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity.
    Croatian Medical Journal. Roč. 57, č. 2 (2016), s. 165-178. ISSN 0353-9504
    Grant CEP: GA ČR(CZ) GC16-01128J
    Institucionální podpora: RVO:61389013
    Klíčová slova: core-shell maghemite nanoparticles * proliferative activity of lymphocytes * phagocytic activity
    Kód oboru RIV: CD - Makromolekulární chemie
    Impakt faktor: 1.619, rok: 2016
    http://www.cmj.hr/2016/57/2/27106358.htm

    To determine cytotoxicity and effect of silica-coated magnetic nanoparticles (MNPs) on immune response, in particular lymphocyte proliferative activity, phagocytic activity, and leukocyte respiratory burst and in vitro production of interleukin-6 (IL-6) and 8 (IL-8), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and granulocyte macrophage colony stimulating factor (GM-CSF). Maghemite was prepared by coprecipitation of iron salts with ammonia, oxidation with NaOCl and modified by tetramethyl orthosilicate and aminosilanes. Particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and X-ray photoelectron spectroscopy (XPS). Cytotoxicity and lymphocyte proliferative activity were assessed using [H-3]-thymidine incorporation into DNA of proliferating human peripheral blood cells. Phagocytic activity and leukocyte respiratory burst were measured by flow cytometry; cytokine levels in cell supernatants were determined by ELISA. The cytotoxicity and in vitro immunotoxicity were found to be minimal in the newly developed porous core-shell gamma-Fe2O3&SiO2-NH2 magnetic nanoparticles.
    Trvalý link: http://hdl.handle.net/11104/0258835