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Advances in Wheat Genetics: From Genome to Field
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SYSNO ASEP 0451398 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Development of the BAC Physical Maps of Wheat Chromosome 6B for Its Genomic Sequencing Tvůrce(i) Kobayashi, A. (JP)
Katagiri, S. (JP)
Karasawa, W. (JP)
Takumi, S. (JP)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Ogihara, Y. (JP)
Handa, H. (JP)Celkový počet autorů 24 Zdroj.dok. Advances in Wheat Genetics: From Genome to Field. - Verlag : Springer, 2015 / Handa H. - ISBN 978-4-431-55674-9 Rozsah stran s. 101-107 Poč.str. 7 s. Poč.výt. 2500 Poč.str.knihy 445 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. JP - Japonsko Klíč. slova genome sequencing ; wheat ; BAC chromosomes Vědní obor RIV EB - Genetika a molekulární biologie Institucionální podpora UEB-Q - RVO:61389030 DOI 10.1007/978-4-431-55675-6_11 Anotace For a purpose of better understanding the genome structure of wheat and accelerating the development of DNA markers for gene isolations and breeding, the Japanese research group, as a member of The International Wheat Genome Sequencing Consortium, is now conducting the physical mapping and genomic sequencing of wheat chromosome 6B of ‘Chinese Spring’ (CS). BAC libraries were constructed respectively using the short and long arm-specific DNAs extracted from the flow-sorted chromosome 6BS and 6BL of double ditelosomic 6B lines of CS. With a sequence-based finger printing method and contig assembly, the BAC physical maps for the 6BS and 6BL have been successfully established. For validation and chromosomal landing of the BAC contigs, we have developed a large number of 6B-specific DNA markers using the public resources including available EST databases, and 6B survey sequence. In parallel, three reference maps, a highresolution radiation hybrid map, a gametocidal system-derived deletion map and a genetic linkage map, are also under construction for anchoring the BAC contigs to their specific genomic regions. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2016
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