Advances in Wheat Genetics: From Genome to Field

0451398 - ÚEB 2016 RIV JP eng M - Část monografie knihy
Kobayashi, A. - Katagiri, S. - Karasawa, W. - Takumi, S. - Doležel, Jaroslav - Ogihara, Y. - Handa, H. … celkem 24 autorů
Development of the BAC Physical Maps of Wheat Chromosome 6B for Its Genomic Sequencing.
Advances in Wheat Genetics: From Genome to Field. Verlag: Springer, 2015 - (Handa, H.), s. 101-107. ISBN 978-4-431-55674-9
Institucionální podpora: RVO:61389030
Klíčová slova: genome sequencing * wheat * BAC chromosomes
Kód oboru RIV: EB - Genetika a molekulární biologie

For a purpose of better understanding the genome structure of wheat and accelerating the development of DNA markers for gene isolations and breeding, the Japanese research group, as a member of The International Wheat Genome Sequencing Consortium, is now conducting the physical mapping and genomic sequencing of wheat chromosome 6B of ‘Chinese Spring’ (CS). BAC libraries were constructed respectively using the short and long arm-specific DNAs extracted from the flow-sorted chromosome 6BS and 6BL of double ditelosomic 6B lines of CS. With a sequence-based finger printing method and contig assembly, the BAC physical maps for the 6BS and 6BL have been successfully established. For validation and chromosomal landing of the BAC contigs, we have developed a large number of 6B-specific DNA markers using the public resources including available EST databases, and 6B survey sequence. In parallel, three reference maps, a highresolution radiation hybrid map, a gametocidal system-derived deletion map and a genetic linkage map, are also under construction for anchoring the BAC contigs to their specific genomic regions.
Trvalý link: http://hdl.handle.net/11104/0252572