Počet záznamů: 1

Biooxidation of 2-phenylethanol to phenylacetic acid by whole-cell Gluconobacter oxydans biocatalyst immobilized in polyelectrolyte complex capsules

SYSNO ASEP	0446563
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Biooxidation of 2-phenylethanol to phenylacetic acid by whole-cell Gluconobacter oxydans biocatalyst immobilized in polyelectrolyte complex capsules
Tvůrce(i)	Bertóková, A. (SK) Vikartovská, A. (SK) Bučko, M. (SK) Gemeiner, P. (SK) Tkáč, J. (SK) Chorvát, D. (SK) Štefuca, V. (SK) Neděla, Vilém (UPT-D)_{RID, ORCID, SAI}
Celkový počet autorů	8
Zdroj.dok.	Biocatalysis and Biotransformation - ISSN 1024-2422 Roč. 33, č. 2 (2015), s. 111-120
Poč.str.	10 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	Gluconobacter oxydans ; natural flavors ; phenylacetic acid ; immobilized whole-cell biocatalyst ; polyelectrolyte complex capsules ; environmental scanning electron microscopy
Vědní obor RIV	JA - Elektronika a optoelektronika, elektrotechnika
CEP	GA14-22777S GA ČR - Grantová agentura ČR
Institucionální podpora	UPT-D - RVO:68081731
UT WOS	000359802000006
EID SCOPUS	84938328523
DOI	10.3109/10242422.2015.1053470
Anotace	A high-performance biocatalyst in the form of encapsulated cells of Gluconobacter oxydans have been developed for production of phenylacetic acid (PAA) as a natural flavor component. Polyelectrolyte complex (PEC) capsules consisting of sodium alginate, cellulose sulfate, poly(methylene-co-guanidine), CaCl2, and NaCl were used for highly controlled and mild encapsulation of cells. Utilization of encapsulated G. oxydans cells was a significant improvement on existing data on operational stability of cells and cumulative product concentration during biocatalytic production of PAA from 2-phenylethanol. Concerning operational stability, encapsulated cells were active over 12 cycles with a high biotransformation rate, while free cells were inactive after 7 cycles of use. The biocatalytic properties of encapsulated G. oxydans were tested in a bubble column reactor over 7 days with a final cumulative product concentration of 25 g/L. High cell viability (90%) was observed within PEC capsules by confocal laser scanning microscopy, performed before and after repetitive PAA production in the bubble column reactor. The surface microstructure of fully hydrated capsules with and without G. oxydans cells was investigated and compared using an environmental scanning electron microscope.
Pracoviště	Ústav přístrojové techniky
Kontakt	Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178
Rok sběru	2016

Počet záznamů: 1