Počet záznamů: 1
Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)
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SYSNO ASEP 0425839 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus) Tvůrce(i) Shaliutina, A. (CZ)
Hulák, M. (CZ)
Li, P. (CZ)
Šulc, Miroslav (MBU-M) RID, ORCID
Dzyuba, B. (CZ)
Linhart, O. (CZ)Zdroj.dok. Reproduction in Domestical Animals. - : Wiley - ISSN 0936-6768
Roč. 48, č. 1 (2013), s. 156-159Poč.str. 4 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova TROUT ONCORHYNCHUS-MYKISS ; SEMEN CHARACTERISTICS ; SPERMATOZOA Vědní obor RIV EE - Mikrobiologie, virologie Institucionální podpora MBU-M - RVO:61388971 UT WOS 000313875800027 DOI 10.1111/j.1439-0531.2012.02118.x Anotace Contents Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p > 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2014
Počet záznamů: 1