Počet záznamů: 1  

Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)

  1. 1. 0425839 - MBU-M 2014 RIV DE eng J - Článek v odborném periodiku
    Shaliutina, A. - Hulák, M. - Li, P. - Šulc, Miroslav - Dzyuba, B. - Linhart, O.
    Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus).
    Reproduction of Domestical Animals. Roč. 48, č. 1 (2013), s. 156-159. ISSN 0936-6768
    Institucionální podpora: RVO:61388971
    Klíčová slova: TROUT ONCORHYNCHUS-MYKISS * SEMEN CHARACTERISTICS * SPERMATOZOA
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 1.177, rok: 2013

    Contents Seminal plasma of sterlet Acipenser ruthenus was evaluated using comparative proteomics to characterize its protein fractions and to determine any influence of multiple sperm collections on these proteins. An experimental group of fish was used, in which sperm was collected three times at 5 h intervals. Protein fractions of seminal plasma were determined by SDS-gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis high-resolution gels (2D). At all stripping times, five protein bands with molecular weights of 93, 53, 48, 33 and 28 kDa were identified using SDS-PAGE. No significant differences (p > 0.05) in relative mass of protein bands among collections were observed. At the third collection, 20 protein spots were detected from the two-dimensional gels, compared to 17 found at the first and second collections. Ten protein spots, from the third stripping, were analysed
    Trvalý link: http://hdl.handle.net/11104/0231673