Počet záznamů: 1

New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II

SYSNO ASEP	0359446
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II
Tvůrce(i)	Jeníková, M. (CZ) Němejc, K. (CZ) Sak, Bohumil (BC-A)_{RID, ORCID} Květoňová, Dana (BC-A)_SAI Kváč, Martin (BC-A)_{RID, RID, ORCID}
Zdroj.dok.	Veterinary Parasitology. - : Elsevier - ISSN 0304-4017 Roč. 176, 2/3 (2011), 120-125
Poč.str.	6 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	Cryptosporidium suis ; Cryptosporidium pig genotype II ; Mixed infection ; Age-specificity ; Species-specific primers
Vědní obor RIV	GJ - Choroby a škůdci zvířat, veterinární medicína
CEP	GP523/07/P117 GA ČR - Grantová agentura ČR
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000289221400004
DOI	10.1016/j.vetpar.2010.11.010
Anotace	Two species of Cryptosporidium are commonly identified in pigs: C. suis and C. pig genotype II. Detection is routinely based on molecular methods such as PCR and subsequent RFLP or gene sequencing. Low sensitivity to mixed infections is solved by designing and testing novel species-specific primers in the present study. Sensitivity of our primers was identical to genus-specific primers, but more (1:48) mixed infections were detected using these species-specific primers on 477 DNA samples originating from naturally infected pigs of different age categories. Our results show differences in age-dependent susceptibility of pigs to the infection. Whereas C. suis was found in all tested categories of pigs (1-12 week of age and sows). Cryptosporidium pig genotype II was recorded only in animals older than 6 week of age. Usage of species-specific primers could help to better the understanding of epidemiology and occurrence (often underestimated) of pig specific Cryptosporidium spp.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2012

Počet záznamů: 1