New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II

0359446 - BC 2012 RIV NL eng J - Článek v odborném periodiku
Jeníková, M. - Němejc, K. - Sak, Bohumil - Květoňová, Dana - Kváč, Martin
New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II.
Veterinary Parasitology. Roč. 176, 2/3 (2011), 120-125. ISSN 0304-4017. E-ISSN 1873-2550
Grant CEP: GA ČR GP523/07/P117
Výzkumný záměr: CEZ:AV0Z60220518
Klíčová slova: Cryptosporidium suis * Cryptosporidium pig genotype II * Mixed infection * Age-specificity * Species-specific primers
Kód oboru RIV: GJ - Choroby a škůdci zvířat, veterinární medicína
Impakt faktor: 2.579, rok: 2011

Two species of Cryptosporidium are commonly identified in pigs: C. suis and C. pig genotype II. Detection is routinely based on molecular methods such as PCR and subsequent RFLP or gene sequencing. Low sensitivity to mixed infections is solved by designing and testing novel species-specific primers in the present study. Sensitivity of our primers was identical to genus-specific primers, but more (1:48) mixed infections were detected using these species-specific primers on 477 DNA samples originating from naturally infected pigs of different age categories. Our results show differences in age-dependent susceptibility of pigs to the infection. Whereas C. suis was found in all tested categories of pigs (1-12 week of age and sows). Cryptosporidium pig genotype II was recorded only in animals older than 6 week of age. Usage of species-specific primers could help to better the understanding of epidemiology and occurrence (often underestimated) of pig specific Cryptosporidium spp.
Trvalý link: http://hdl.handle.net/11104/0197235