Počet záznamů: 1
Role of Ubiquitin C-Terminal Hydrolase-L1 in Antipolyspermy Defense of Mammalian Oocytes
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SYSNO ASEP 0345745 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Role of Ubiquitin C-Terminal Hydrolase-L1 in Antipolyspermy Defense of Mammalian Oocytes Tvůrce(i) Šušor, Andrej (UZFG-Y) RID, ORCID
Lišková, Lucie (UZFG-Y)
Toralová, Tereza (UZFG-Y) ORCID
Pavlok, Antonín (UZFG-Y) RID
Pivoňková, Kateřina (UZFG-Y)
Karabínová, Pavla (UZFG-Y) RID
Lopatářová, M. (CZ)
Šutovský, P. (US)
Kubelka, Michal (UZFG-Y) RID, ORCIDZdroj.dok. Biology of Reproduction. - : Oxford University Press - ISSN 0006-3363
Roč. 82, č. 6 (2010), s. 1151-1161Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cortical granule ; deubiquitinating ; fertilization Vědní obor RIV ED - Fyziologie CEP GA524/07/1087 GA ČR - Grantová agentura ČR GP524/09/P435 GA ČR - Grantová agentura ČR GD204/09/H084 GA ČR - Grantová agentura ČR CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000277964500015 DOI https://doi.org/10.1095/biolreprod.109.081547 Anotace The ubiquitin-proteasome system regulates many cellular processes via proteasomal degradation of ubiquitinated proteins. Ubiquitin C-terminal hydrolase-L1 (UCHL1), an abundant protein in mammalian oocytes, has hydrolytic activity as a monomer. In its dimeric and oligomeric form, UCHL1 acts as an ubiquitin ligase. Recently was found that, deficiency in UCHL1 coincides with polyspermic fertilization; however, the role of UCHL1 in such process remains unclear. After in vitro fertilization, a high rate of polyspermy in bovine zygotes was induced by UCHL1 inhibitors and decreased level of the monomeric as well as polymeric ubiquitin pool was detected. In addition, presumptive UCHL1 oligomers were formed after UCHL1 inhibition with subsequent increase of K63-linked polyubiquitin chains in oocytes. Moreover, both migration of cortical granules (CGs) toward the cortex during oocyte maturation and fertilization-induced extrusion of CGs were impaired after UCHL1 inhibition. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2011
Počet záznamů: 1