Počet záznamů: 1

Role of Ubiquitin C-Terminal Hydrolase-L1 in Antipolyspermy Defense of Mammalian Oocytes

SYSNO ASEP	0345745
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Role of Ubiquitin C-Terminal Hydrolase-L1 in Antipolyspermy Defense of Mammalian Oocytes
Tvůrce(i)	Šušor, Andrej (UZFG-Y)_{RID, ORCID} Lišková, Lucie (UZFG-Y) Toralová, Tereza (UZFG-Y)_ORCID Pavlok, Antonín (UZFG-Y)_RID Pivoňková, Kateřina (UZFG-Y) Karabínová, Pavla (UZFG-Y)_RID Lopatářová, M. (CZ) Šutovský, P. (US) Kubelka, Michal (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Biology of Reproduction. - : Oxford University Press - ISSN 0006-3363 Roč. 82, č. 6 (2010), s. 1151-1161
Poč.str.	11 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	cortical granule ; deubiquitinating ; fertilization
Vědní obor RIV	ED - Fyziologie
CEP	GA524/07/1087 GA ČR - Grantová agentura ČR
	GP524/09/P435 GA ČR - Grantová agentura ČR
	GD204/09/H084 GA ČR - Grantová agentura ČR
CEZ	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000277964500015
DOI	10.1095/biolreprod.109.081547
Anotace	The ubiquitin-proteasome system regulates many cellular processes via proteasomal degradation of ubiquitinated proteins. Ubiquitin C-terminal hydrolase-L1 (UCHL1), an abundant protein in mammalian oocytes, has hydrolytic activity as a monomer. In its dimeric and oligomeric form, UCHL1 acts as an ubiquitin ligase. Recently was found that, deficiency in UCHL1 coincides with polyspermic fertilization; however, the role of UCHL1 in such process remains unclear. After in vitro fertilization, a high rate of polyspermy in bovine zygotes was induced by UCHL1 inhibitors and decreased level of the monomeric as well as polymeric ubiquitin pool was detected. In addition, presumptive UCHL1 oligomers were formed after UCHL1 inhibition with subsequent increase of K63-linked polyubiquitin chains in oocytes. Moreover, both migration of cortical granules (CGs) toward the cortex during oocyte maturation and fertilization-induced extrusion of CGs were impaired after UCHL1 inhibition.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2011

Počet záznamů: 1