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Subunit–subunit interactions are weakened in mutant forms of acetohydroxy acid synthase insensitive to valine inhibition
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SYSNO ASEP 0340830 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Subunit–subunit interactions are weakened in mutant forms of acetohydroxy acid synthase insensitive to valine inhibition Tvůrce(i) Kyselková, Martina (MBU-M)
Janata, Jiří (MBU-M) RID, ORCID
Ságová-Marečková, M. (CZ)
Kopecký, J. (CZ)Zdroj.dok. Archives of Microbiology. - : Springer - ISSN 0302-8933
Roč. 192, č. 3 (2010), s. 195-200Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Streptomyces cinnamonensis ; Acetohydroxy acid synthase ; Subunit-subunit interaction Vědní obor RIV EE - Mikrobiologie, virologie CEP 2B08064 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000274455600006 DOI 10.1007/s00203-010-0545-0 Anotace In acetohydroxy acid synthase from Streptomyces cinnamonensis mutants aVected in valine regulation, the impact of mutations on interactions between the catalytic and the regulatory subunits was examined using yeast twohybrid system. Mutations in the catalytic and the regulatory subunits were projected into homology models of the respective proteins. Two changes in the catalytic subunit, E139A (domain) and Q217 (domain), both located on the surface of the catalytic subunit dimer, lowered the interaction with the regulatory subunit. Three consecutive changes in the N-terminal part of the regulatory subunit were examined Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2010
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