Počet záznamů: 1
Subunit–subunit interactions are weakened in mutant forms of acetohydroxy acid synthase insensitive to valine inhibition
- 1.0340830 - MBU-M 2010 RIV DE eng J - Článek v odborném periodiku
Kyselková, Martina - Janata, Jiří - Ságová-Marečková, M. - Kopecký, J.
Subunit–subunit interactions are weakened in mutant forms of acetohydroxy acid synthase insensitive to valine inhibition.
Archives of Microbiology. Roč. 192, č. 3 (2010), s. 195-200. ISSN 0302-8933
Grant CEP: GA MŠk 2B08064
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: Streptomyces cinnamonensis * Acetohydroxy acid synthase * Subunit-subunit interaction
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 1.754, rok: 2010
In acetohydroxy acid synthase from Streptomyces cinnamonensis mutants aVected in valine regulation, the impact of mutations on interactions between the catalytic and the regulatory subunits was examined using yeast twohybrid system. Mutations in the catalytic and the regulatory subunits were projected into homology models of the respective proteins. Two changes in the catalytic subunit, E139A (domain) and Q217 (domain), both located on the surface of the catalytic subunit dimer, lowered the interaction with the regulatory subunit. Three consecutive changes in the N-terminal part of the regulatory subunit were examined
Trvalý link: http://hdl.handle.net/11104/0183993