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Viability staining and animal infectivity of Cryptosporidium andersoni oocysts after long-term storage
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SYSNO ASEP 0090744 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Viability staining and animal infectivity of Cryptosporidium andersoni oocysts after long-term storage Překlad názvu Životaschopnost a infektivita oocyst Cryptosporidium andersoni při dlouhodobém skladování Tvůrce(i) Kváč, Martin (BC-A) RID, RID, ORCID
Květoňová, Dana (BC-A) SAI
Salát, Jiří (BC-A) RID, ORCID
Ditrich, Oleg (BC-A) ORCIDZdroj.dok. Parasitology Research. - : Springer - ISSN 0932-0113
Roč. 100, č. 2 (2007), s. 213-217Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Cryptosporidium andersoni ; viability ; infectivity ; long-term storage Vědní obor RIV EG - Zoologie CEP GA524/05/0992 GA ČR - Grantová agentura ČR CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) Anotace Preservation of biological properties of oocysts during the storage is critical for experimental work. Stomach cryptosporidia species are less resistant against external influences and their infectivity decreases rapidly in comparison with intestinal species. C. andersoni oocysts lost their infectivity for gerbil after 7 months storage in deionised water (DW) or in 2.5% potassium dichromate solution (PDS). Evaluation of oocyst viability by flow cytometry indicates higher percentage of viable oocysts stored in PDS than in DW, particularly after 6 months of storage. However, direct counting using fluorescent microscope revealed that these results are false and are influenced by the change of staining properties during the storage in PDS. Moreover, the examination of oocyst integrity by flow cytometry revealed that oocysts preserved in PDS kept their wall integrity longer than those stored in DW, and this fact should be taken into consideration during quantification of oocyst survival. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2008
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