Origin of cells cultured in vitro from human breast carcinomas traced by cyclin D1 and HER2/neu FISH signal numbers
1.
SYSNO ASEP
0001378
Druh ASEP
J - Článek v odborném periodiku
Zařazení RIV
J - Článek v odborném periodiku
Poddruh J
Ostatní články
Název
Origin of cells cultured in vitro from human breast carcinomas traced by cyclin D1 and HER2/neu FISH signal numbers
Překlad názvu
Původ buněk kultivovaných in vitro z buněk lidského karcinomu prsu zjišťovaný počtem signálů FISH pro cyklin D1 a HER2/neu
Tvůrce(i)
Matoušková, Eva (UMG-J) Kudláčková, Iva (UMG-J) Chaloupková, Alena (UMG-J) Brožová, Markéta (UMG-J) Netíková, I. (CZ) Veselý, Pavel (UMG-J)
Zdroj.dok.
Anticancer Research. - : International Institute of Anticancer Research
- ISSN 0250-7005
Roč. 25, 2A (2005), s. 1051-1058
Poč.str.
8 s.
Jazyk dok.
eng - angličtina
Země vyd.
GR - Řecko
Klíč. slova
breast carcinomas ; primary cultures of carcinoma cells ; cyclin D1 and HER2/neu by FISH
Vědní obor RIV
EB - Genetika a molekulární biologie
CEP
NR8145 GA MZd - Ministerstvo zdravotnictví
CEZ
AV0Z50520514 - UMG-J (2005-2011)
Anotace
We investigated if malignant cells in situ additionally characterized by increased numbers of proto-oncogenes can be traced by the FISH method in the ex vivo-derived cultures. In 6 tumors (5 primary tumors, 1 cutaneous metastasis), increased numbers of FISH signals were found in 55-99% of cells. In cell populations maintained for up to 2-6 passages in vitro the incidence of cells with increased FISH signals was found to be low (2-16%). Moreover, the cells with multiplied signals that survived more than one passage in vitro were evidently unable to divide further. However, in all 6 tumors at least a small fraction of cells displaying only two signals of CCND1 or HER2/neu genes was identified directly in invasive tumor structures in the vicinity of cells with multiple signals. Our findings suggest that these invasive tumor cells displaying only two proto-oncogene signals were most probably involved in the initiation and propagation of ex vivo tumor-derived primary cell cultures.
