Počet záznamů: 1
Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension
- 1.0555843 - FGÚ 2023 RIV US eng J - Článek v odborném periodiku
Dahale, S. - Ruiz-Orera, J. - Šilhavý, Jan - Hübner, N. - van Heesch, S. - Pravenec, Michal - Atanur, S. S.
Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension.
Life Science Alliance. Roč. 5, č. 4 (2022), č. článku e202101234. E-ISSN 2575-1077
Grant ostatní: AV ČR(CZ) AP1502
Program: Akademická prémie - Praemium Academiae
Institucionální podpora: RVO:67985823
Klíčová slova: cap analysis of gene expression (CAGE) * alternative promoter usage * spontaneously hypertensive rat * left ventricle
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.400, rok: 2022
Způsob publikování: Open access
The role of alternative promoter usage in tissue-specific gene expression has been well established, however, its role in complex diseases is poorly understood. We performed cap analysis of gene expression (CAGE) sequencing from the left ventricle of a rat model of hypertension, the spontaneously hypertensive rat (SHR), and a normotensive strain, Brown Norway to understand the role of alternative promoter usage in complex disease. We identified 26,560 CAGE-defined transcription start sites in the rat left ventricle, including 1,970 novel cardiac transcription start sites. We identified 28 genes with alternative promoter usage between SHR and Brown Norway, which could lead to protein isoforms differing at the amino terminus between two strains and 475 promoter switching events altering the length of the 5′ UTR. We found that the shift in Insr promoter usage was significantly associated with insulin levels and blood pressure within a panel of HXB/BXH recombinant inbred rat strains, suggesting that hyperinsulinemia due to insulin resistance might lead to hypertension in SHR. Our study provides a preliminary evidence of alternative promoter usage in complex diseases.
Trvalý link: http://hdl.handle.net/11104/0330305
Počet záznamů: 1