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Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal
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SYSNO ASEP 0524280 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal Tvůrce(i) Ujčíková, Hana (FGU-C) RID, ORCID
Cechová, Kristína (FGU-C) RID, ORCID
Jágr, Michal (FGU-C) RID, ORCID
Roubalová, Lenka (FGU-C) RID, ORCID, SAI
Vošahlíková, Miroslava (FGU-C) RID, ORCID, SAI
Svoboda, Petr (FGU-C) RID, ORCIDČíslo článku e0231721 Zdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 15, č. 4 (2020)Poč.str. 25 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova morphine withdrawal ; rat hippocampus ; 2D-DIGE ; label-free quantification ; immunoblot analysis ; MALDI-TOF MS/MS Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA17-05903S GA ČR - Grantová agentura ČR GA19-03295S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 UT WOS 000529957800001 EID SCOPUS 85083327650 DOI 10.1371/journal.pone.0231721 Anotace Opioid addiction is recognized as a chronic relapsing brain disease resulting from repeated exposure to opioid drugs. Cellular and molecular mechanisms underlying the ability of organism to return back to the physiological norm after cessation of drug supply are not fully understood. The aim of this work was to extend our previous studies of morphine-induced alteration of rat forebrain cortex protein composition to the hippocampus. Rats were exposed to morphine for 10 days and sacrificed 24 h (groups +M10 and -M10) or 20 days after the last dose of morphine (groups +M10/-M20 and -M10/-M20). The six altered proteins (>= 2-fold) were identified in group (+M10) when compared with group (-M10) by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). The number of differentially expressed proteins was increased to thirteen after 20 days of the drug withdrawal. Noticeably, the altered level of alpha-synuclein, beta-synuclein, alpha-enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was also determined in both (+/- M10) and (+/- M10/-M20) samples of hippocampus. Immunoblot analysis of 2D gels by specific antibodies oriented against alpha/beta-synucleins and GAPDH confirmed the data obtained by 2D-DIGE analysis. Label-free quantification identified nineteen differentially expressed proteins in group (+M10) when compared with group (-M10). After 20 days of morphine withdrawal (+/- M10/-M20), the number of altered proteins was increased to twenty. We conclude that the morphine-induced alteration of protein composition in rat hippocampus after cessation of drug supply proceeds in a different manner when compared with the forebrain cortex. In forebrain cortex, the total number of altered proteins was decreased after 20 days without morphine, whilst in hippocampus, it was increased. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2021 Elektronická adresa https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231721
Počet záznamů: 1