Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal

0524280 - FGÚ 2021 RIV US eng J - Článek v odborném periodiku
Ujčíková, Hana - Cechová, Kristína - Jágr, Michal - Roubalová, Lenka - Vošahlíková, Miroslava - Svoboda, Petr
Proteomic analysis of protein composition of rat hippocampus exposed to morphine for 10 days, comparison with animals after 20 days of morphine withdrawal.
PLoS ONE. Roč. 15, č. 4 (2020), č. článku e0231721. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA ČR(CZ) GA17-05903S; GA ČR(CZ) GA19-03295S
Institucionální podpora: RVO:67985823
Klíčová slova: morphine withdrawal * rat hippocampus * 2D-DIGE * label-free quantification * immunoblot analysis * MALDI-TOF MS/MS
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.240, rok: 2020
Způsob publikování: Open access
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0231721

Opioid addiction is recognized as a chronic relapsing brain disease resulting from repeated exposure to opioid drugs. Cellular and molecular mechanisms underlying the ability of organism to return back to the physiological norm after cessation of drug supply are not fully understood. The aim of this work was to extend our previous studies of morphine-induced alteration of rat forebrain cortex protein composition to the hippocampus. Rats were exposed to morphine for 10 days and sacrificed 24 h (groups +M10 and -M10) or 20 days after the last dose of morphine (groups +M10/-M20 and -M10/-M20). The six altered proteins (>= 2-fold) were identified in group (+M10) when compared with group (-M10) by two-dimensional fluorescence difference gel electrophoresis (2D-DIGE). The number of differentially expressed proteins was increased to thirteen after 20 days of the drug withdrawal. Noticeably, the altered level of alpha-synuclein, beta-synuclein, alpha-enolase and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was also determined in both (+/- M10) and (+/- M10/-M20) samples of hippocampus. Immunoblot analysis of 2D gels by specific antibodies oriented against alpha/beta-synucleins and GAPDH confirmed the data obtained by 2D-DIGE analysis. Label-free quantification identified nineteen differentially expressed proteins in group (+M10) when compared with group (-M10). After 20 days of morphine withdrawal (+/- M10/-M20), the number of altered proteins was increased to twenty. We conclude that the morphine-induced alteration of protein composition in rat hippocampus after cessation of drug supply proceeds in a different manner when compared with the forebrain cortex. In forebrain cortex, the total number of altered proteins was decreased after 20 days without morphine, whilst in hippocampus, it was increased.
Trvalý link: http://hdl.handle.net/11104/0308651