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Mouse oocytes nucleoli rescue embryonic development of porcine enucleolated oocytes
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SYSNO ASEP 0486664 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Mouse oocytes nucleoli rescue embryonic development of porcine enucleolated oocytes Tvůrce(i) Morovic, M. (SK)
Strejček, F. (SK)
Nakagawa, S. (CZ)
Deshmukh, R.S. (IN)
Murin, M. (SK)
Benc, M. (SK)
Fulka, Helena (UMG-J)
Kyogoku, H. (JP)
Pendovski, L. (MK)
Fulka, J. (CZ)
Laurinčik, Jozef (UZFG-Y)Celkový počet autorů 11 Zdroj.dok. Zygote. - : Cambridge University Press - ISSN 0967-1994
Roč. 25, č. 6 (2017), s. 675-685Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Embryo ; Interspecies nucleolar transfer ; Mouse ; Nucleolus ; Olcytes ; Pig Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Reproductive biology (medical aspects to be 3) CEP EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMG-J - RVO:68378050 ; UZFG-Y - RVO:67985904 UT WOS 000418839900002 DOI https://doi.org/10.1017/S0967199417000491 Anotace It is well known that nucleoli of fully grown mammalian oocytes are indispensable for embryonic development. Therefore, the embryos originated from previously enucleolated (ENL) oocytes undergo only one or two cleavages and then their development ceases. In our study the interspecies (mouse/pig) nucleolus transferred embryos (NuTE) were produced and their embryonic development was analyzed by autoradiography, transmission electron microscopy (TEM) and immunofluorescence (C23 and upstream binding factor (UBF)). Our results show that the re-injection of isolated oocyte nucleoli, either from the pig (P + P) or mouse (P + M), into previously enucleolated and subsequently matured porcine oocytes rescues their development after parthenogenetic activation and some of these develop up to the blastocyst stage (P + P, 11.8%, P + M, 13.5%). In nucleolus re-injected 8-cell and blastocyst stage embryos the number of nucleoli labeled with C23 in P + P and P + M groups was lower than in control (non-manipulated) group. UBF was localized in small foci within the nucleoli of blastocysts in control and P + P embryos, however, in P + M embryos the labeling was evenly distributed in the nucleoplasm. The TEM and autoradiographic evaluations showed the formation of functional nucleoli and de novo rRNA synthesis at the 8-cell stage in both, control and P + P group. In the P + M group the formation of comparable nucleoli was delayed. In conclusion, our results indicate that the mouse nucleolus can rescue embryonic development of enucleolated porcine oocytes, but the localization of selected nucleolar proteins, the timing of transcription activation and the formation of the functional nucleoli in NuTE compared with control group show evident aberrations. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2018
Počet záznamů: 1