Počet záznamů: 1  

Mouse oocytes nucleoli rescue embryonic development of porcine enucleolated oocytes

  1. 1.
    0486664 - ÚMG 2018 RIV GB eng J - Článek v odborném periodiku
    Morovic, M. - Strejček, F. - Nakagawa, S. - Deshmukh, R.S. - Murin, M. - Benc, M. - Fulka, Helena - Kyogoku, H. - Pendovski, L. - Fulka, J. - Laurinčik, Jozef
    Mouse oocytes nucleoli rescue embryonic development of porcine enucleolated oocytes.
    Zygote. Roč. 25, č. 6 (2017), s. 675-685. ISSN 0967-1994. E-ISSN 1469-8730
    Grant CEP: GA MŠMT EF15_003/0000460
    Institucionální podpora: RVO:68378050 ; RVO:67985904
    Klíčová slova: Embryo * Interspecies nucleolar transfer * Mouse * Nucleolus * Olcytes * Pig
    Obor OECD: Reproductive biology (medical aspects to be 3); Developmental biology (UZFG-Y)
    Impakt faktor: 1.114, rok: 2017

    It is well known that nucleoli of fully grown mammalian oocytes are indispensable for embryonic development. Therefore, the embryos originated from previously enucleolated (ENL) oocytes undergo only one or two cleavages and then their development ceases. In our study the interspecies (mouse/pig) nucleolus transferred embryos (NuTE) were produced and their embryonic development was analyzed by autoradiography, transmission electron microscopy (TEM) and immunofluorescence (C23 and upstream binding factor (UBF)). Our results show that the re-injection of isolated oocyte nucleoli, either from the pig (P + P) or mouse (P + M), into previously enucleolated and subsequently matured porcine oocytes rescues their development after parthenogenetic activation and some of these develop up to the blastocyst stage (P + P, 11.8%, P + M, 13.5%). In nucleolus re-injected 8-cell and blastocyst stage embryos the number of nucleoli labeled with C23 in P + P and P + M groups was lower than in control (non-manipulated) group. UBF was localized in small foci within the nucleoli of blastocysts in control and P + P embryos, however, in P + M embryos the labeling was evenly distributed in the nucleoplasm. The TEM and autoradiographic evaluations showed the formation of functional nucleoli and de novo rRNA synthesis at the 8-cell stage in both, control and P + P group. In the P + M group the formation of comparable nucleoli was delayed. In conclusion, our results indicate that the mouse nucleolus can rescue embryonic development of enucleolated porcine oocytes, but the localization of selected nucleolar proteins, the timing of transcription activation and the formation of the functional nucleoli in NuTE compared with control group show evident aberrations.
    Trvalý link: http://hdl.handle.net/11104/0281406

     
     
Počet záznamů: 1  

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