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Surface N-glycoproteome patterns reveal key proteins of neuronal differentiation
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SYSNO ASEP 0458488 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Surface N-glycoproteome patterns reveal key proteins of neuronal differentiation Tvůrce(i) Tylečková, Jiřina (UZFG-Y)
Valeková, Ivona (UZFG-Y) RID, ORCID
Žižková, Martina (UZFG-Y) RID
Rákocyová, Michaela (UZFG-Y)
Maršala, S. (US)
Maršala, M. (US)
Gadher, S. J. (US)
Kovářová, Hana (UZFG-Y) RID, ORCIDZdroj.dok. Journal of Proteomics. - : Elsevier - ISSN 1874-3919
Roč. 132, č. 1 (2016), s. 13-20Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova cell adhesion proteins ; cell surface capture ; neuronal differentiation Vědní obor RIV EB - Genetika a molekulární biologie CEP ED2.1.00/03.0124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy TA01011466 GA TA ČR - Technologická agentura ČR Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000368747400002 EID SCOPUS 84969630129 DOI 10.1016/j.jprot.2015.11.008 Anotace Pluripotent stem cell-derived committed neural precursors are an important source of cells to treat neurodegenerative diseases including spinal cord injury. There remains an urgency to identify markers for monitoring of neural progenitor specificity, estimation of neural fate and follow-up correlation with therapeutic effect in preclinical studies using animal disease models. Cell surface capture technology was used to uncover the cell surface exposed N-glycoproteome of neural precursor cells upon neuronal differentiation as well as post-mitotic mature hNT neurons. The data presented depict an extensive study of surfaceome during neuronal differentiation, confirming glycosylation at a particular predicted site of many of the identified proteins. Quantitative changes detected in cell surface protein levels reveal a set of proteins that highlight the complexity of the neuronal differentiation process. Several of these proteins including the cell adhesion molecules ICAM1, CHL1, and astrotactinl as well as LAMPI were validated by SRM. Combination of immunofluorescence staining of ICAM1 and flow cytometry indicated a possible direction for future scrutiny of such proteins as targets for enrichment of the neuronal subpopulation from mixed cultures after differentiation of neural precursor cells. These surface proteins hold an important key for development of safe strategies in cell-replacement therapies of neuronal disorders. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2017
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