Počet záznamů: 1  

Surface N-glycoproteome patterns reveal key proteins of neuronal differentiation

  1. 1.
    0458488 - UZFG-Y 2017 RIV NL eng J - Článek v odborném periodiku
    Tylečková, Jiřina - Valeková, Ivona - Žižková, Martina - Rákocyová, Michaela - Maršala, S. - Maršala, M. - Gadher, S. J. - Kovářová, Hana
    Surface N-glycoproteome patterns reveal key proteins of neuronal differentiation.
    Journal of Proteomics. Roč. 132, č. 1 (2016), s. 13-20. ISSN 1874-3919
    Grant CEP: GA MŠk ED2.1.00/03.0124; GA TA ČR(CZ) TA01011466
    Institucionální podpora: RVO:67985904
    Klíčová slova: cell adhesion proteins * cell surface capture * neuronal differentiation
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.914, rok: 2016

    Pluripotent stem cell-derived committed neural precursors are an important source of cells to treat neurodegenerative diseases including spinal cord injury. There remains an urgency to identify markers for monitoring of neural progenitor specificity, estimation of neural fate and follow-up correlation with therapeutic effect in preclinical studies using animal disease models. Cell surface capture technology was used to uncover the cell surface exposed N-glycoproteome of neural precursor cells upon neuronal differentiation as well as post-mitotic mature hNT neurons. The data presented depict an extensive study of surfaceome during neuronal differentiation, confirming glycosylation at a particular predicted site of many of the identified proteins. Quantitative changes detected in cell surface protein levels reveal a set of proteins that highlight the complexity of the neuronal differentiation process. Several of these proteins including the cell adhesion molecules ICAM1, CHL1, and astrotactinl as well as LAMPI were validated by SRM. Combination of immunofluorescence staining of ICAM1 and flow cytometry indicated a possible direction for future scrutiny of such proteins as targets for enrichment of the neuronal subpopulation from mixed cultures after differentiation of neural precursor cells. These surface proteins hold an important key for development of safe strategies in cell-replacement therapies of neuronal disorders.
    Trvalý link: http://hdl.handle.net/11104/0258756