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Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato
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SYSNO ASEP 0438490 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato Tvůrce(i) Albacete, A. (AT)
Cantero-Navarro, E. (ES)
Grosskinsky, D. K. (AT)
Arias, M.L. (AR)
Balibrea, M. E. (ES)
Bru, R. (ES)
Fragner, L. (AT)
Ghanem, M. E. (ES)
de la Cruz Gonzalez, M. (ES)
Hernández, J. A. (ES)
Martínez-Andújar, C. (ES)
van der Graaff, E. (AT)
Weckwerth, W. (AT)
Zellnig, G. (AT)
Pérez-Alfocea, F. (ES)
Roitsch, Thomas (UEK-B) RID, ORCID, SAIZdroj.dok. Journal of Experimental Botany. - : Oxford University Press - ISSN 0022-0957
Roč. 66, č. 3 (2015), s. 863-878Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Cell wall invertase ; cytokinins ; drought stress ; ethylene ; source–sink relationships ; tomato Vědní obor RIV EH - Ekologie - společenstva Institucionální podpora RVO:67179843 - RVO:67179843 UT WOS 000351662300021 EID SCOPUS 84928169025 DOI https://doi.org/10.1093/jxb/eru448 Anotace Drought stress conditions modify source-sink relations, thereby influencing plant growth, adaptive responses, and consequently crop yield. Invertases are key metabolic enzymes regulating sink activity through the hydrolytic cleavage of sucrose into hexose monomers, thus playing a crucial role in plant growth and development. However, the physiological role of invertases during adaptation to abiotic stress conditions is not yet fully understood. Here it is shown that plant adaptation to drought stress can be markedly improved in tomato (Solanum lycopersicum L.) by overexpression of the cell wall invertase (cwInv) gene CIN1 from Chenopodium rubrum. CIN1 overexpression limited stomatal conductance under normal watering regimes, leading to reduced water consumption during the drought period, while photosynthetic activity was maintained. This caused a strong increase in water use efficiency (up to 50%), markedly improving water stress adaptation through an efficient physiological strategy of dehydration avoidance. Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants. However, the CIN1-overexpressing plants registered 3- to 6-fold higher cwInv activity in all analysed conditions. Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation. The induced sink metabolism in the leaves explained the maintenance of photosynthetic activity, delayed senescence, and increased source activity under drought stress. Moreover, CIN1 plants also presented a better control of production of reactive oxygen species and sustained membrane protection. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2016
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