0438490 - ÚVGZ 2016 RIV GB eng J - Článek v odborném periodiku
Albacete, A. - Cantero-Navarro, E. - Grosskinsky, D. K. - Arias, M.L. - Balibrea, M. E. - Bru, R. - Fragner, L. - Ghanem, M. E. - de la Cruz Gonzalez, M. - Hernández, J. A. - Martínez-Andújar, C. - van der Graaff, E. - Weckwerth, W. - Zellnig, G. - Pérez-Alfocea, F. - Roitsch, Thomas
Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato.
Journal of Experimental Botany. Roč. 66, č. 3 (2015), s. 863-878. ISSN 0022-0957. E-ISSN 1460-2431
Institucionální podpora: RVO:67179843
Klíčová slova: Cell wall invertase * cytokinins * drought stress * ethylene * source–sink relationships * tomato
Kód oboru RIV: EH - Ekologie - společenstva
Impakt faktor: 5.677, rok: 2015

Drought stress conditions modify source-sink relations, thereby influencing plant growth, adaptive responses, and consequently crop yield. Invertases are key metabolic enzymes regulating sink activity through the hydrolytic cleavage of sucrose into hexose monomers, thus playing a crucial role in plant growth and development. However, the physiological role of invertases during adaptation to abiotic stress conditions is not yet fully understood. Here it is shown that plant adaptation to drought stress can be markedly improved in tomato (Solanum lycopersicum L.) by overexpression of the cell wall invertase (cwInv) gene CIN1 from Chenopodium rubrum. CIN1 overexpression limited stomatal conductance under normal watering regimes, leading to reduced water consumption during the drought period, while photosynthetic activity was maintained. This caused a strong increase in water use efficiency (up to 50%), markedly improving water stress adaptation through an efficient physiological strategy of dehydration avoidance. Drought stress strongly reduced cwInv activity and induced its proteinaceous inhibitor in the leaves of the wild-type plants. However, the CIN1-overexpressing plants registered 3- to 6-fold higher cwInv activity in all analysed conditions. Surprisingly, the enhanced invertase activity did not result in increased hexose concentrations due to the activation of the metabolic carbohydrate fluxes, as reflected by the maintenance of the activity of key enzymes of primary metabolism and increased levels of sugar-phosphate intermediates under water deprivation. The induced sink metabolism in the leaves explained the maintenance of photosynthetic activity, delayed senescence, and increased source activity under drought stress. Moreover, CIN1 plants also presented a better control of production of reactive oxygen species and sustained membrane protection.
Trvalý link: http://hdl.handle.net/11104/0241938