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Der p 11 Is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis
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SYSNO ASEP 0436615 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Der p 11 Is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis Tvůrce(i) Banerjee, S. (AT)
Resch, Y. (AT)
Chen, K. W. (AT)
Swoboda, I. (AT)
Focke-Tejkl, M. (AT)
Blatt, K. (AT)
Novak, N. (DE)
Wickman, M. (SE)
van Hage, M. (SE)
Ferrara, R. (IT)
Mari, A. (IT)
Purohit, A. (FR)
Pauli, G. (FR)
Sibanda, E. N. (ZW)
Ndlovu, P. (ZW)
Thomas, W. R. (AU)
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Tacke, S. (AT)
Malkus, U. (DE)
Valent, P. (AT)
Valenta, R. (AT)
Vrtala, S. (AT)Celkový počet autorů 22 Zdroj.dok. Journal of Investigative Dermatology - ISSN 0022-202X
Roč. 135, č. 1 (2015), s. 102-109Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova allergen ; house dust mites ; immunogold electron microscopy Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika CEP LO1212 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UPT-D - RVO:68081731 UT WOS 000346225000016 EID SCOPUS 84925228067 DOI https://doi.org/10.1038/jid.2014.271 Anotace House dust mites (HDMs) belong to the most potent indoor allergen sources worldwide and are associated with allergic manifestations in the respiratory tract and the skin. Here we studied the importance of the high-molecular-weight group 11 allergen from Dermatophagoides pteronyssinus (Der p 11) in HDM allergy. Sequence analysis showed that Der p 11 has high homology to paramyosins from mites, ticks, and other invertebrates. A synthetic gene coding for Der p 11 was expressed in Escherichia coli and rDer p 11 purified to homogeneity as folded, alpha-helical protein as determined by circular dichroism spectroscopy. Using antibodies raised against rDer p 11 and immunogold electron microscopy, the allergen was localized in the muscle beneath the skin of mite bodies but not in feces. IgE reactivity of rDer p 11 was tested with sera from HDM-allergic patients from Europe and Africa in radioallergosorbent test–based dot-blot assays. Interestingly, we found that Der p 11 is a major allergen for patients suffering from atopic dermatitis (AD), whereas it is only a minor allergen for patients suffering from respiratory forms of HDM allergy. Thus, rDer p 11 might be a useful serological marker allergen for the identification of a subgroup of HDM-allergic patients suffering from HDM-associated AD. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2015
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