0436615 - ÚPT 2015 RIV US eng J - Článek v odborném periodiku
Banerjee, S. - Resch, Y. - Chen, K. W. - Swoboda, I. - Focke-Tejkl, M. - Blatt, K. - Novak, N. - Wickman, M. - van Hage, M. - Ferrara, R. - Mari, A. - Purohit, A. - Pauli, G. - Sibanda, E. N. - Ndlovu, P. - Thomas, W. R. - Krzyžánek, Vladislav - Tacke, S. - Malkus, U. - Valent, P. - Valenta, R. - Vrtala, S.
Der p 11 Is a Major Allergen for House Dust Mite-Allergic Patients Suffering from Atopic Dermatitis.
Journal of Investigative Dermatology. Roč. 135, č. 1 (2015), s. 102-109. ISSN 0022-202X. E-ISSN 1523-1747
Grant CEP: GA MŠMT(CZ) LO1212
Institucionální podpora: RVO:68081731
Klíčová slova: allergen * house dust mites * immunogold electron microscopy
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika
Impakt faktor: 6.915, rok: 2015

House dust mites (HDMs) belong to the most potent indoor allergen sources worldwide and are associated with allergic manifestations in the respiratory tract and the skin. Here we studied the importance of the high-molecular-weight group 11 allergen from Dermatophagoides pteronyssinus (Der p 11) in HDM allergy. Sequence analysis showed that Der p 11 has high homology to paramyosins from mites, ticks, and other invertebrates. A synthetic gene coding for Der p 11 was expressed in Escherichia coli and rDer p 11 purified to homogeneity as folded, alpha-helical protein as determined by circular dichroism spectroscopy. Using antibodies raised against rDer p 11 and immunogold electron microscopy, the allergen was localized in the muscle beneath the skin of mite bodies but not in feces. IgE reactivity of rDer p 11 was tested with sera from HDM-allergic patients from Europe and Africa in radioallergosorbent test–based dot-blot assays. Interestingly, we found that Der p 11 is a major allergen for patients suffering from atopic dermatitis (AD), whereas it is only a minor allergen for patients suffering from respiratory forms of HDM allergy. Thus, rDer p 11 might be a useful serological marker allergen for the identification of a subgroup of HDM-allergic patients suffering from HDM-associated AD.
Trvalý link: http://hdl.handle.net/11104/0240378