0427792 - ÚFCH JH 2015 RIV NL eng J - Journal Article
Štefl, Martin - Šachl, Radek - Olžyńska, Agnieszka - Amaro, Mariana Manuela - Savchenko, Dariia - Deyneka, Alexander - Hermetter, A. - Cwiklik, Lukasz - Humpolíčková, Jana - Hof, Martin
Comprehensive portrait of cholesterol containing oxidized membrane.
Biochimica Et Biophysica Acta-Biomembranes. Roč. 1838, č. 7 (2014), s. 1769-1776. ISSN 0005-2736. E-ISSN 1879-2642
R&D Projects: GA ČR GBP208/12/G016; GA MŠMT LH13259
Grant - others:SAFMAT(CZ) CZ.2. 13/3.1.00/22132
Institutional support: RVO:61388955 ; RVO:68378271
Keywords : oxidized lipids * lateral diffusion * molecular dynamics simulations
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 3.836, year: 2014 ; AIS: 1.257, rok: 2014
DOI: https://doi.org/10.1016/j.bbamem.2014.02.006

Biologicalmembranes are under significant oxidative stress caused by reactive oxygen species mostly originating during cellular respiration. Double bonds of the unsaturated lipids are most prone to oxidation,which might lead to shortening of the oxidized chain and inserting of terminal either aldehyde or carboxylic group. Structural rearrangement of oxidized lipids, addressed already, is mainly associated with looping back of the hydrophilic terminal group. This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Particularly, attention is paid to cholesterol rearrangement in the oxidized membrane revealing its preferable interaction with carbonyls of the oxidized chains. In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer.
Permanent Link: http://hdl.handle.net/11104/0233275