Comprehensive portrait of cholesterol containing oxidized membrane

0427792 - ÚFCH JH 2015 RIV NL eng J - Článek v odborném periodiku
Štefl, Martin - Šachl, Radek - Olžyńska, Agnieszka - Amaro, Mariana - Savchenko, Dariia - Deyneka, Alexander - Hermetter, A. - Cwiklik, Lukasz - Humpolíčková, Jana - Hof, Martin
Comprehensive portrait of cholesterol containing oxidized membrane.
Biochimica Et Biophysica Acta-Biomembranes. Roč. 1838, č. 7 (2014), s. 1769-1776. ISSN 0005-2736. E-ISSN 1879-2642
Grant CEP: GA ČR GBP208/12/G016; GA MŠk LH13259
Grant ostatní: SAFMAT(CZ) CZ.2. 13/3.1.00/22132
Institucionální podpora: RVO:61388955 ; RVO:68378271
Klíčová slova: oxidized lipids * lateral diffusion * molecular dynamics simulations
Kód oboru RIV: CF - Fyzikální chemie a teoretická chemie
Impakt faktor: 3.836, rok: 2014

Biologicalmembranes are under significant oxidative stress caused by reactive oxygen species mostly originating during cellular respiration. Double bonds of the unsaturated lipids are most prone to oxidation,which might lead to shortening of the oxidized chain and inserting of terminal either aldehyde or carboxylic group. Structural rearrangement of oxidized lipids, addressed already, is mainly associated with looping back of the hydrophilic terminal group. This contribution utilizing dual-focus fluorescence correlation spectroscopy and electron paramagnetic resonance as well as atomistic molecular dynamics simulations focuses on the overall changes of the membrane structural and dynamical properties once it becomes oxidized. Particularly, attention is paid to cholesterol rearrangement in the oxidized membrane revealing its preferable interaction with carbonyls of the oxidized chains. In this view cholesterol seems to have a tendency to repair, rather than condense, the bilayer.
Trvalý link: http://hdl.handle.net/11104/0233275