Počet záznamů: 1
Analysis of plant galactolipids by reversed-phase high-performance liquid chromatography/mass spectrometry with accurate mass measurement
- 1.
SYSNO ASEP 0381415 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of plant galactolipids by reversed-phase high-performance liquid chromatography/mass spectrometry with accurate mass measurement Author(s) Zábranská, Marie (UOCHB-X)
Vrkoslav, Vladimír (UOCHB-X) RID, ORCID
Sobotníková, J. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCIDNumber of authors 4 Source Title Chemistry and Physics of Lipids. - : Elsevier - ISSN 0009-3084
Roč. 165, č. 5 (2012), s. 601-607Number of pages 7 s. Language eng - English Country IE - Ireland Keywords accurate mass measurement ; DGDG ; equivalent carbon number ; MGDG ; RP-HPLC/MS Subject RIV CB - Analytical Chemistry, Separation R&D Projects GA203/09/0139 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000308681700011 DOI https://doi.org/10.1016/j.chemphyslip.2012.03.002 Annotation The composition of plant membrane lipids was investigated by reversed-phase high performance liquid chromatography mass spectrometry with accurate mass measurement. The data dependent methods for the analysis of monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) have been developed. The optimised chromatographic systems were based on a 2.0 mm i.d. Nucleosil C18 column with methanol/water (MGDGs) or acetonitrile/methanol/water (DGDGs) gradients. The galactolipids were ionised by electrospray operated in the positive ion mode and identified based on their MS/MS spectra. High resolution spectra with accurate masses were found to be essential for correct interpretation of the MS data. The elution order of non-oxidised MGDGs and DGDGs followed the equivalent carbon numbers. The methods were applied for detailed characterisation of the MGDGs and DGDGs in the leaves of Arabidopsis thaliana and Melissa officinalis. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2013
Počet záznamů: 1