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Analysis of plant galactolipids by reversed-phase high-performance liquid chromatography/mass spectrometry with accurate mass measurement

  1. 1.
    0381415 - ÚOCHB 2013 RIV IE eng J - Článek v odborném periodiku
    Zábranská, Marie - Vrkoslav, Vladimír - Sobotníková, J. - Cvačka, Josef
    Analysis of plant galactolipids by reversed-phase high-performance liquid chromatography/mass spectrometry with accurate mass measurement.
    Chemistry and Physics of Lipids. Roč. 165, č. 5 (2012), s. 601-607. ISSN 0009-3084. E-ISSN 1873-2941
    Grant CEP: GA ČR GA203/09/0139
    Grant ostatní: GA UK(CZ) SVV 2012-265201
    Výzkumný záměr: CEZ:AV0Z40550506
    Klíčová slova: accurate mass measurement * DGDG * equivalent carbon number * MGDG * RP-HPLC/MS
    Kód oboru RIV: CB - Analytická chemie, separace
    Impakt faktor: 2.147, rok: 2012

    The composition of plant membrane lipids was investigated by reversed-phase high performance liquid chromatography mass spectrometry with accurate mass measurement. The data dependent methods for the analysis of monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) have been developed. The optimised chromatographic systems were based on a 2.0 mm i.d. Nucleosil C18 column with methanol/water (MGDGs) or acetonitrile/methanol/water (DGDGs) gradients. The galactolipids were ionised by electrospray operated in the positive ion mode and identified based on their MS/MS spectra. High resolution spectra with accurate masses were found to be essential for correct interpretation of the MS data. The elution order of non-oxidised MGDGs and DGDGs followed the equivalent carbon numbers. The methods were applied for detailed characterisation of the MGDGs and DGDGs in the leaves of Arabidopsis thaliana and Melissa officinalis.
    Trvalý link: http://hdl.handle.net/11104/0211891

     
     
Počet záznamů: 1  

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