Počet záznamů: 1
Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by lnositol 3,4,5,6-Tetrakisphosphate
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SYSNO ASEP 0172243 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by lnositol 3,4,5,6-Tetrakisphosphate Tvůrce(i) Zonia, Laura (UEB-Q)
Cordeiro, S. (PT)
Tupý, Jaroslav (UEB-Q) RID
Feijó, J. (PT)Zdroj.dok. Plant Cell. - : Oxford University Press - ISSN 1040-4651
Roč. 14, - (2002), s. 2233-2249Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Volume Regulation ; Chloride Efflux ; lnositol Vědní obor RIV EF - Botanika CEP LN00A081 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z5038910 - UEB-Q Anotace Oscillatory growth of pollen tubes has been correlated with oscillatory influxes of the cations Ca2+, H+, and K+. Using an ion-specific vibrating probe, a new circuit was identified that involves oscillatory efflux of the anion Cl- at the apex and steady influx along the tube starting at 12 (j.m distal to the tip. This spatial coupling of influx and efflux sites predicts that a vectorial flux of Cl ion traverses the apical region. The Cl channel blockers 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and 5-nitro-2-(3-phenylpropylamino)benzoic acid completely inhibited tobacco pollen tube growth at 80 and 20 (xM, respectively. Cl channel blockers also induced increases in apical cell volume. The apical 50 (Jim of untreated pollen tubes had a mean cell volume of 3905 75 xm3. DIDS at 80 j.M caused a rapid and lethal cell volume increase to 6206 +- 171 (j.m3, which is at the point of cell bursting at the apex. DIDS was further demonstrated to disrupt Cl efflux from the apex, indicating that Cl flux correlates with pollen tube growth and cell volume status. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2003
Počet záznamů: 1