0172243 - UEB-Q 20023023 RIV US eng J - Článek v odborném periodiku
Zonia, Laura - Cordeiro, S. - Tupý, Jaroslav - Feijó, J.
Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by lnositol 3,4,5,6-Tetrakisphosphate.
Plant Cell. Roč. 14, - (2002), s. 2233-2249. ISSN 1040-4651. E-ISSN 1532-298X
Grant CEP: GA MŠMT LN00A081
Výzkumný záměr: CEZ:AV0Z5038910
Klíčová slova: Volume Regulation * Chloride Efflux * lnositol
Kód oboru RIV: EF - Botanika
Impakt faktor: 10.751, rok: 2002

Oscillatory growth of pollen tubes has been correlated with oscillatory influxes of the cations Ca2+, H+, and K+. Using an ion-specific vibrating probe, a new circuit was identified that involves oscillatory efflux of the anion Cl- at the apex and steady influx along the tube starting at 12 (j.m distal to the tip. This spatial coupling of influx and efflux sites predicts that a vectorial flux of Cl ion traverses the apical region. The Cl channel blockers 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and 5-nitro-2-(3-phenylpropylamino)benzoic acid completely inhibited tobacco pollen tube growth at 80 and 20 (xM, respectively. Cl channel blockers also induced increases in apical cell volume. The apical 50 (Jim of untreated pollen tubes had a mean cell volume of 3905 75 xm3. DIDS at 80 j.M caused a rapid and lethal cell volume increase to 6206 +- 171 (j.m3, which is at the point of cell bursting at the apex. DIDS was further demonstrated to disrupt Cl efflux from the apex, indicating that Cl flux correlates with pollen tube growth and cell volume status.
Trvalý link: http://hdl.handle.net/11104/0069282