Lysozyme from the gut of the soft tick Ornithodoros moubata: the sequence, phylogeny and post-feeding regulation
1.
SYSNO ASEP
0157623
Druh ASEP
J - Článek v odborném periodiku
Zařazení RIV
J - Článek v odborném periodiku
Poddruh J
Ostatní články
Název
Lysozyme from the gut of the soft tick Ornithodoros moubata: the sequence, phylogeny and post-feeding regulation
Tvůrce(i)
Grunclová, L. (CZ) Fouquier, H. (CZ) Hypša, Václav (PAU-O) Kopáček, Petr (PAU-O)
Zdroj.dok.
Developmental and Comparative Immunology. - : Elsevier
- ISSN 0145-305X
Roč. 27, č. 8 (2003), s. 651-660
Poč.str.
10 s.
Jazyk dok.
eng - angličtina
Země vyd.
GB - Velká Británie
Klíč. slova
Ornithodoros moubata ; lysozyme ; innate immunity
Vědní obor RIV
EB - Genetika a molekulární biologie
CEP
GA206/00/0266 GA ČR - Grantová agentura ČR
CEZ
AV0Z6022909 - PAU-O, BC-A
MSM 123100003
Anotace
Sequence of a tick gut lysozyme (TGL) from the soft tick Ornithodoros moubata was determined by cloning and sequencing of overlapping polymerase chain reaction (PCR) and RACE PCR products. It is the first lysozyme sequence representing the subphylum Chelicerata. The resulting open reading frame codes for a putative signal peptide of 22 amino-acid residues and a mature protein composed of 124 amino-acids. Calculated mass of the protein is 14037.75 Da and a theoretical isoelectric point is 8.16. The phylogenetic analysis revealed that the TGL belongs to the c-type lysozymes. It forms a distinct monophyletic group together with multiple lysozyme-like sequences found in the gene products agCP6542 from Anopheles gambiae strain PEST and CG8492-PA from Drosophila melanogaster. TGL seems to be an interesting case in which the features of lysozymes with anti-bacterial and digestive function are combined.
