0157623 - PAU-O 20033010 RIV GB eng J - Článek v odborném periodiku
Grunclová, L. - Fouquier, H. - Hypša, Václav - Kopáček, Petr
Lysozyme from the gut of the soft tick Ornithodoros moubata: the sequence, phylogeny and post-feeding regulation.
Developmental and Comparative Immunology. Roč. 27, č. 8 (2003), s. 651-660. ISSN 0145-305X. E-ISSN 1879-0089
Grant CEP: GA ČR GA206/00/0266
Výzkumný záměr: CEZ:AV0Z6022909; CEZ:MSM 123100003
Klíčová slova: Ornithodoros moubata * lysozyme * innate immunity
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.390, rok: 2003

Sequence of a tick gut lysozyme (TGL) from the soft tick Ornithodoros moubata was determined by cloning and sequencing of overlapping polymerase chain reaction (PCR) and RACE PCR products. It is the first lysozyme sequence representing the subphylum Chelicerata. The resulting open reading frame codes for a putative signal peptide of 22 amino-acid residues and a mature protein composed of 124 amino-acids. Calculated mass of the protein is 14037.75 Da and a theoretical isoelectric point is 8.16. The phylogenetic analysis revealed that the TGL belongs to the c-type lysozymes. It forms a distinct monophyletic group together with multiple lysozyme-like sequences found in the gene products agCP6542 from Anopheles gambiae strain PEST and CG8492-PA from Drosophila melanogaster. TGL seems to be an interesting case in which the features of lysozymes with anti-bacterial and digestive function are combined.
Trvalý link: http://hdl.handle.net/11104/0055061