Počet záznamů: 1  

Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis

    1.
    SYSNO ASEP0136110
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JOstatní články
    NázevUse of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis
    Tvůrce(i) Uhlířová, Miroslava (ENTU-I)
    Foy, B. D. (US)
    Beaty, B. J. (US)
    Olson, K. E. (US)
    Riddiford, L. M. (US)
    Jindra, Marek (ENTU-I)
    Zdroj.dok.Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424
    Roč. 100, č. 26 (2003), s. 15607-15612
    Poč.str.6 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaGreen fluorescent protein-steroid-hormone ecdysone
    Vědní obor RIVEB - Genetika a molekulární biologie
    CEPIAA5007305 GA AV ČR - Akademie věd
    CEZAV0Z5007907 - ENTU-I, BC-A
    AnotaceThe transcription factor Broad-Complex (BR-C) is required for differentiation of adult structures as well as for the programmed death of obsolete larval organs during metamorphosis of the fruit fly Drosophila melanogaster. Whether BR-C has a similar role in other holometabolous insects could not be proven without a loss-of-function genetic test, performed in a non-drosophilid species. Here we use a recombinant Sindbis virus as a tool to silence BR-C expression in the silkmoth Bombyx mori. The virus expressing a BR-C antisense RNA fragment reduced endogenous BR-C mRNA levels in infected tissues (adult wing and leg primordia) via RNA interference (RNAi). The RNAi knock-down of BR-C resulted in the failure of animals to complete the larval-pupal transition or in later morphogenetic defects, including differentiation of adult compound eyes, legs, and wings from their larval progenitors. BR-C RNAi also perturbed the programmed cell death of larval silk glands. These developmental defects correspond to loss-of-function phenotypes of BR-C Drosophila mutants in both the morphogenetic and degenerative aspects, suggesting that the critical role of BR-C in metamorphosis is evolutionarily conserved. We also demonstrate that the Sindbis virus is a useful vehicle for silencing of developmental genes in new insect models.
    PracovištěEntomologický ústav (do r. 2005)
    KontaktHelena Musilová, musilova@entu.cas.cz, Tel.: 387 775 216
    Rok sběru2004

Počet záznamů: 1  

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